Ana Leite Oliveira

Centre for Biotechnology and Fine Chemistry (CBQF)

Ana Leite Oliveira

Ana Leite Oliveira is the head of the Biomaterials and Biomedical Technology Laboratory at CBQF and Assistant Professor with Habilitation at Escola Superior de Biotecnologia, Universidade Católica Portuguesa, where she is also the Director of the Master of Biomedical Engineering. She holds a PhD from the University of Minho (2008), collaborating with Depuy Orthopaedics, Inc. (Johnson&Johnson), USA and CSIC, Madrid, followed by a Post-Doc with Tufts University, Boston, USA. She gained expertise in natural-based biomaterials for tissue regeneration, with a focus on skin-related applications. Recently, she is also been driven to experiment new processing routes to modulate biological materials’ biofunctionality and ensure its safeness, with the perspective of subsequent scaling-up. She has published over 90 international peer reviewed articles and book chapters and participated in more than 100 international scientific and technological conferences, mostly as an oral presenter or as an invited lecturer. The innovation in her work generated 6 patents and links to industry, working with companies to reach new products/processes. She successfully prepared/coordinated various projects funded by National/European agencies and private companies. Since 2008 she has been assisting the European Commission as an Independent Expert and she participates in several boards and interest groups.


Publications (2019-2023)



Oliveira AL, Veiga A, Bernardes B, Costa JB, Rojo del Olmo L. Off-The-Shelf Silk Sericin; Methods And Uses Thereof, Patent nr 2023231000146 (07.06.23).

Oliveira AL, Ribeiro VP, Rosadas M, Silva IV, Sousa AC, Reis A, Figueiredo, RN, Skin Substitutes, Methods And Uses Thereof, Patent nr 20232005106224 (30.05.23).

Oliveira AL, Silva SB, Borges SC, Alves PJ, Silk Sericin-based Hydrogel, Methods and Uses Thereof, Patent number 20161000045461 (2016); WO/2018/011732 (2018).

San Roman J, Vasquez BM, Garcia LF, Barbosa AM, Gomes F, Oliveira AL. Activators for the crosslinking of polysaccharides and proteins with antioxidant activity, Patent number P201730794 (2018).


Papers in international peer-reviewed journals:

Silva IV, Sanna M, Duarte MM, Costa R, Ribeiro VP, Rodrigues I, Mendes-Ferreira P, Soares R, Moroni L, Oliveira AL*. Impact of Supercritical Carbon Dioxide as a Decellularization Agent for Aortic Tissue, Biomaterials, in submission.

Rosadas M, Silva IV, Costa JB, Ribeiro VP, Oliveira AL*, Decellularized Dermal Matrices: Unleashing the potential in Tissue Engineering and Regenerative Medicine. Frontiers in Materials, submitted.

Veiga A, Foster O, Kaplan D, Oliveira AL*, Expanding the Boundaries of silk-sericin biomaterials in Biomedical Applications, Materials Today Bio, submitted.

Veiga A, Madureira S, Costa JB, Castro F, Rocha F, Oliveira AL*. Tackling current production of HAp and HAp-driven biomaterials. Materials Advances. 2023.

Guimarães I, Costa R, Madureira S, Borges S, Oliveira AL, Pintado M, Baptista-Silva S. Tannic Acid Tailored-Made Microsystems for Wound Infection. International Journal of Molecular Sciences. 2023 Mar 2;24(5):4826.

Singh S, Pereira J, Brandão T, Oliveira AL, Poças F. Recycling of polypropylene by supercritical carbon dioxide for extraction of contaminants from beverage cups. A comparison with polyethylene terephthalate and polylactic acid. Journal of the Science of Food and Agriculture. 2023 Feb;103(3):1127-38.

Alves PJ, Elg F, Bothma G, Oliveira AL, Neves-Amado J, Amado J, Shifting the distribution curve for healthcare resource use through topical oxygen therapy for wound healing, Journal of Wound Care. Feb 2022 vol.31, 3, (IF 4.947)

Duarte MM, Silva IV, Eisenhut AR, Bionda N, Duarte AR, Oliveira AL*. Contributions of supercritical fluid technology for advancing decellularization and postprocessing of viable biological materials. Materials Horizons. 2022;9(3):864-91. (IF 14.356) . (IF 14.356)

Veiga A, Magalhães R, Duarte MM, Dias JR, Alves NM, Costa-Pinto AR, Castro F, Rocha F, Oliveira AL*. Continuous Production of Highly Tuned Silk/Calcium-Based Composites: Exploring New Pathways for Skin Regeneration. Molecules. 2022 Mar 30;27(7):2249. (IF: 4,927)

Baptista-Silva S, Bernardes BG, Borges S, Rodrigues I, Fernandes R, Gomes-Guerreiro S, Pinto MT, Pintado M, Soares R, Costa R, Oliveira AL*. Exploring Silk Sericin for Diabetic Wounds: An In Situ-Forming Hydrogel to Protect against Oxidative Stress and Improve Tissue Healing and Regeneration. Biomolecules. 2022 Jun;12(6):801. (IF: 4,569)

Zou S, Fan S, Oliveira AL, Yao X, Zhang Y, Shao H. 3D Printed Gelatin Scaffold with Improved Shape Fidelity and Cytocompatibility by Using Antheraea pernyi Silk Fibroin Nanofibers. Advanced Fiber Materials. 2022 Mar 1:1-6. (IF: 12,958)

Pérez-Davila S, González-Rodríguez L, Lama R, López-Álvarez M, Oliveira AL, Serra J, Novoa B, Figueras A, González P. 3D-Printed PLA Medical Devices: Physicochemical Changes and Biological Response after Sterilisation Treatments. Polymers. 2022 Oct 1;14(19):4117. (IF: 4,329)

Veiga A, Castro F, Ferreira A, Oliveira AL*, Rocha F. Fabrication of calcium phosphates with controlled properties using a modular oscillatory flow reactor. Chemical Engineering Research and Design. 2022 Jul 1;183:90-103. (IF: 3.739)

Singh S, Pereira J, Brandão T, Oliveira AL, Poças F. Recycling of polypropylene by supercritical carbon dioxide for extraction of contaminants from beverage cups. A comparison with polyethylene terephthalate and polylactic acid. Journal of the Science of Food and Agriculture. 2022 Sep 29. (IF:3,639)

Veiga A, Silva IV, Duarte MM and Oliveira AL*, Current trends on protein driven bioinks for 3D printing, Pharmaceutics. 2021 Pharmaceutics 2021, 13(9), 1444; (IF: 6,321)

Bernardes BG, Del Gaudio P, Alves P, Costa R, García-Gonzaléz CA, Oliveira AL*. Bioaerogels: Promising Nanostructured Materials in Fluid Management, Healing and Regeneration of Wounds. Molecules. 2021; 26(13):3834. (IF 3.267).

Baptista-Silva S, Borges S, Costa-Pinto AR, Costa R, Amorim M, Dias JR, Ramos Oì, Alves P, Granja PL, Soares R, Pintado M, Oliveira AL*. In Situ Forming Silk Sericin-Based Hydrogel: A Novel Wound Healing Biomaterial. ACS Biomaterials Science and Engineering. 2021; 7, 4; 10.1021/acsbiomaterials.0c01745 (IF 4.152).

Duarte MM, Ribeiro N, Silva I V., Dias JR, Alves NM, Oliveira AL*. Fast decellularization process using supercritical carbon dioxide for trabecular bone. Journal of Supercritical Fluids. 2021; 172, 105194; 10.1016/j.supflu.2021.105194 (IF 3.744).

Guimarães I, Baptista-Silva S, Pintado M, Oliveira AL*. Polyphenols: A promising avenue in therapeutic solutions for wound care. Applied Sciences (Switzerland). 2021; 11, 3, 1230; 10.3390/app11031230 (IF 2.474).

Veiga A, Castro F, Oliveira AL*, Rocha F. High efficient strategy for the production of hydroxyapatite/silk sericin nanocomposites. Journal of Chemical Technology & Biotechnology. 2021; 96, 1, 241–8. 10.1002/jctb.6532 (IF 2.750).

Fernández-Villa D, Asensio G, Silva M, Ramírez-Jiménez RA, Saldaña L, Vilaboa N, Oliveira AL, San Román J, Vázquez-Lasa B, Rojo L. Vitamin B9 derivatives as carriers of bioactive cations for musculoskeletal regeneration applications: Synthesis, characterization and biological evaluation. European Journal of Medicinal Chemistry. 2021; 212, 113152; 10.1016/j.ejmech.2021.113152 (IF 5.572).

Vieira E, Silva M, Maia-Filho A, Ferreira D, Figuerêdo-Silva J, Rovaris K, Fialho AC, Oliveira AL, de Oliveira ALM, da Fonseca MG, Saima JA, da Silva-Filho EC. Effect of cerium-containing hydroxyapatite in bone repair in female rats with osteoporosis induced by ovariectomy. Minerals. 2021; 11(4), 377; 10.3390/min11040377 (IF 2.380).

Ribeiro N, Sousa A, Cunha-Reis C, Oliveira AL, Granja PL, Monteiro FJ, Sousa SR. New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers. Nanomedicine: Nanotechnology, Biology and Medicine. 2021; 33, 102353; 10.1016/j.nano.2020.102353 (IF 5.182).

Silva SC, Braz EM, Brito CA, Alves MM, Carvalho FA, Barreto HM, Oliveira AL, Silva DA, Silva-Filho EC. Phthalic anhydride esterified chicha gum: characterization and antibacterial activity. Carbohydrate Polymers. 2021; 251, 117077; 10.1016/j.carbpol.2020.117077 (IF 7.182).

Braz EM, Silva SC, Brito CA, Carvalho FA, Alves MM, Barreto HM, Silva DA, Magalhães R, Oliveira AL, Silva-Filho EC. Modified chicha gum by acetylation for antimicrobial and antiparasitic applications: Characterization and biological properties. International Journal of Biological Macromolecules. 2020; 160, 1177-1188; 10.1016/j.ijbiomac.2020.05.219 (IF 5.162).

Ribeiro N, Soares GC, Santos-Rosales V, Concheiro A, Alvarez-Lorenzo C, García-González CA, Oliveira AL*. A new era for sterilization based on supercritical CO2 technology. Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2020; 108(2), 399-428; 10.1002/jbm.b.34398 (IF 2.831).

Mora-Boza A, García-Fernández L, Barbosa FA, Oliveira AL, Vázquez-Lasa B, San Román J. Glycerylphytate crosslinker as a potential osteoinductor of chitosan-based systems for guided bone regeneration. Carbohydrate Polymers. 2020; 241, 116269; 10.1016/j.carbpol.2020.116269 (IF 7.182).

Dias JR, Ribeiro N, Baptista-Silva S, Costa-Pinto AR, Alves N, Oliveira AL*. In situ Enabling Approaches for Tissue Regeneration: Current Challenges and New Developments. Frontiers in Bioengineering and Biotechnology. 2020; 8, 85; 10.3389/fbioe.2020.00085 (IF 3.644).

Veiga A, Castro F, Rocha F, Oliveira AL*. Protein-Based Hydroxyapatite Materials: Tuning Composition toward Biomedical Applications. ACS Applied Bio Materials. 2020; 3, 6, 3441–55. 10.1021/acsabm.0c00140 (IF 2.57).

Best MG, Cunha-Reis C, Ganin AY, Sousa A, Johnston J, Oliveira AL, Smith DGE, Yiu HHP, Cooper IR. Antimicrobial Properties of Gallium(III)- and Iron(III)-Loaded Polysaccharides Affecting the Growth of Escherichia coli , Staphylococcus aureus, and Pseudomonas aeruginosa , In Vitro. ACS Applied Bio Materials. 2020; 3, 11, 7589–97. 10.1021/acsabm.0c00811 (IF 2.57).

Veiga A, Castro F, Rocha F, Oliveira AL*. Silk‐based microcarriers: current developments and future perspectives. IET Nanobiotechnology. 2020; 14, 8, 645–53. 10.1049/iet-nbt.2020.0058

Santos MV, Oliveira AL, Osajima JA, Silva-Filho EC. Development of composites scaffolds with calcium and cerium-hydroxyapatite and gellan gum. Ceramics International. 2020; 46, 3, 3811–7. 10.1016/j.ceramint.2019.10.104 (IF 3.830).

Veiga A, Castro F, Rocha F, Oliveira AL*. Recent Advances in Silk Sericin/Calcium Phosphate Biomaterials. Frontiers in Materials. 2020; 7; 10.3389/fmats.2020.00024 (IF 2.705).

Veiga A, Castro F, Reis CC, Sousa A, Oliveira AL*, Rocha F. Hydroxyapatite/sericin composites: A simple synthesis route under near-physiological conditions of temperature and pH and preliminary study of the effect of sericin on the biomineralization process. Materials Science and Engineering: C. 2020; 108, 110400. 10.1016/j.msec.2019.110400 (IF 5.880).

Ribeiro VP, Pina S, Costa JB, Cengiz IF, García-Fernández L, Fernández-Gutiérrez MDM, Paiva OC, Oliveira AL, San-Román J, Oliveira JM, Reis RL. Enzymatically Cross-Linked Silk Fibroin-Based Hierarchical Scaffolds for Osteochondral Regeneration. ACS Applied Materials and Interfaces. 2019; 11, 4, 3781-3799; 10.1021/acsami.8b21259 (IF 8.758).

Geão C, Costa-Pinto AR, Cunha-Reis C, Ribeiro VP, Vieira S, Oliveira JM, Reis RL, Oliveira AL*. Thermal annealed silk fibroin membranes for periodontal guided tissue regeneration. Journal of Materials Science: Materials in Medicine. 2019; 30, 2, 2710.1007/s10856-019-6225-y (IF 2.489).

Soares GC, Learmonth DA, Vallejo MC, Davila SP, González P, Sousa RA, Oliveira AL*. Supercritical CO2 technology: The next standard sterilization technique? Materials Science and Engineering C. 2019; 99, 520-540; 10.1016/j.msec.2019.01.121 (IF 5.880).

Serôdio R, Schickert SL, Costa-Pinto AR, Dias JR, Granja PL, Yang F, Oliveira AL*. Ultrasound sonication prior to electrospinning tailors silk fibroin/PEO membranes for periodontal regeneration. Materials Science and Engineering: C. 2019; 98, 969–81. 10.1016/j.msec.2019.01.055 (IF 5.880).

Santos-Rosales V, Ardao I, Alvarez-Lorenzo C, Ribeiro N, Oliveira AL, García-González C. Sterile and Dual-Porous Aerogels Scaffolds Obtained through a Multistep Supercritical CO2-Based Approach. Molecules. 2019; 24, 5, 871. 10.3390/molecules24050871 (IF 3.267).

Santos MV, Rocha LB Vieira EG, Oliveira AL, Lobo AO, Carvalho MA, Osajima JA, Silva-Filho EC. Development of Composite Scaffolds Based on Cerium Doped-Hydroxyapatite and Natural Gums—Biological and Mechanical Properties. Materials. 2019; 12, 15, 2389. 10.3390/ma12152389 (IF 3.057).

Ribeiro VP, da Silva Morais A, Maia FR, Canadas RF, Costa JB, Oliveira AL, Oliveira JM, Reis RL. Combinatory approach for developing silk fibroin scaffolds for cartilage regeneration. Acta Biomaterialia. 2018; 10.1016/j.actbio.2018.03.047 (IF 6.638).

Dias JR, Baptista-Silva S, Sousa A, Oliveira AL, Bártolo PJ, Granja PL. Biomechanical performance of hybrid electrospun structures for skin regeneration. Materials Science and Engineering: C. 2018; 93, 816–27; 10.1016/j.msec.2018.08.050 (IF 4.959).

Ribeiro VP, Silva-Correia J, Gonçalves C, Pina S, Radhouani H, Montonen T, Hyttinen J, Roy A, Oliveira AL, Reis RL, Oliveira JM. Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death. PLOS ONE. 2018; 13, 4, e0194441; 10.1371/journal.pone.0194441 (IF 2.776).


Research Lab: Biomaterials and Biomedical Technology


Assistant Professor

Academic CV